This enables the cells to freeze at approximately -one°C/minute, that's perfect for freezing most cell varieties. You can even choose to make use of a controlled fee freezer and keep the vials specifically at -80°C.

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Aseptic approaches: Use appropriate aseptic procedures to keep up sterile conditions although freezing your cells. It is a good follow to wipe down the skin of containers, for example flasks and freezing media vials, with 70% ethanol or isopropanol in advance of opening them.

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Proper storage: Go with solitary-use, sterile cryogenic vials for storing your cells. It truly is preferable to work with interior-threaded cryogenic vials to forestall contamination through filling or when stored in liquid nitrogen. Locate sterile cryogenic vials >

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Contamination-totally free cells: Previous to freezing, ensure that the cells are wholesome and free of any microbial contamination.